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tert sequencing  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc tert sequencing
    Tert Sequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tert sequencing/product/Pyrosequencing Inc
    Average 90 stars, based on 1 article reviews
    tert sequencing - by Bioz Stars, 2026-04
    90/100 stars

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    Image Search Results


    FluoroDetect assay—Mechanism of action (A) Schematic workflow of the FluoroDetect assay. Fluorescence signal of mCherry reporter gene can be downregulated by siRNA while the green fluorescence signal of the EGFP remains stable. (B) DNA and mRNA sequence of the target motif used in the FluoroDetect C-rich (HSat3) assay containing the flanking enzymatic cutting sites (pink) and siRNA-binding sites (blue). Additionally, the siRNA and ASO sequences are shown (m: 2′ MeO modification, ∗: thiophosphate modification). (C) Proof of concept using HeLa cells. Fluorescence microscope images showing green and red fluorescence signal in samples treated with targeting siRNA (siHSat3), untargeted siRNA control pool (siCo), and an untreated control. Images were taken 48 h after transfection.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: A dual fluorescence-based reporter assay for real-time determination of siRNA- and antisense oligonucleotide-mediated knockdown

    doi: 10.1016/j.omtn.2025.102631

    Figure Lengend Snippet: FluoroDetect assay—Mechanism of action (A) Schematic workflow of the FluoroDetect assay. Fluorescence signal of mCherry reporter gene can be downregulated by siRNA while the green fluorescence signal of the EGFP remains stable. (B) DNA and mRNA sequence of the target motif used in the FluoroDetect C-rich (HSat3) assay containing the flanking enzymatic cutting sites (pink) and siRNA-binding sites (blue). Additionally, the siRNA and ASO sequences are shown (m: 2′ MeO modification, ∗: thiophosphate modification). (C) Proof of concept using HeLa cells. Fluorescence microscope images showing green and red fluorescence signal in samples treated with targeting siRNA (siHSat3), untargeted siRNA control pool (siCo), and an untreated control. Images were taken 48 h after transfection.

    Article Snippet: A transfer plasmid coding only EGFP was cloned by inserting an EGFP sequence into the pCDH-3xFLAG-TERT (Addgene plasmid #51631, RRID: Addgene 51631) generously provided by Steven Artandi.

    Techniques: Fluorescence, Sequencing, Binding Assay, Modification, Microscopy, Control, Transfection